Start Submission Become a Reviewer

Reading: In-vitro establishment, germination and growth performance of Red Sandalwood (Pterocarpus sa...

Download

A- A+
Alt. Display

Research Articles

In-vitro establishment, germination and growth performance of Red Sandalwood (Pterocarpus santalinus L.)

Authors:

G. D. G. Chaturani ,

University of Ruhuna, Mapalana, Kamburupitiya, LK
About G. D. G.
Department of Crop Science, Faculty of Agriculture
X close

S. Subasinghe,

University of Ruhuna, Mapalana, Kamburupitiya, LK
About S.
Department of Crop Science, Faculty of Agriculture
X close

M. P. Jayatilleke

University of Ruhuna, Mapalana, Kamburupitiya, LK
About M. P.
Department of Crop Science, Faculty of Agriculture
X close

Abstract

For the selection of a suitable surface sterilization procedure for in-vitro establishment of seeds, 0.1 % Mercuric Chloride was used with five different exposure times (5, 10, 15, 20, and 25 min). Pods with their highest external diameter of < 3, 3-4, 4-5, and > 5 cm were used to examine the effect of pod size on in-vitro germination and pods har-vested at light brown stage and, stored at ambient temperature (28 ±2 °C) for 1, 2, 3, and 4 weeks, were used to examine the effect of storage time on in-vitro germination. To study the effect of different culture conditions on in-vitro germination, seeds were cultured on hormone free Murashige & Skoog (1962), Anderson (1980), Vitis (1987), and Woody Plant Medium (1980) with (1 g/l) and without activated charcoal. To study the in-vitro growth performance of red sandalwood seedlings, germinated seeds were cultured on hormone free MS, Anderson, and Woody Plant Medium at their full strength incorporated with 1 g/l of activated charcoal. Dipping the pods in 0.1 % HgCl2 with 2-3 drops of Tween 20 for fifteen minutes was suitable for surface sterilization. Pods with <3 cm of diameter did not contain viable seeds. Seeds obtained from pods with >4 cm in diameter showed a significantly higher germination (90 %) ability, while time taken for germination was not affected by pod diameter. Success of seed germination was reduced with increasing storage time. Seeds cultured within one week of harvest showed the highest germination (96 %) and short germination time (6 days), while seeds cultured after 4 weeks showed relatively less germination (61 %) and longer germination time (10 days). Germination percentage was significantly higher (92%) in Anderson medium without charcoal, and low germination percentages of 62, 62 and 61 % were recorded on seeds cultured on woody plant medium (with charcoal), MS and Vitis medium (without charcoal), respectively. Seeds cultured on Anderson, Vitis and woody plant medium took almost similar time (6-8 days) for germination. Seeds cultured on Vitis medium (with charcoal) showed the longest hypocotyls length, 13.8 mm. The highest plant height of 9.9 cm and highest number of nodes per seedling (7 nodes/shoot) were recorded for seedlings in woody plant medium while MS medium showed the lowest number of nodes (6 nodes/shoot) and lowest plant height of 7.3 cm. Leaf formation was best in woody plant medium, and the average leaf diameter is 10.8 mm. The root system developed well in all media. The highest mean score for the appearance of the plant was recorded in woody plant medium (3.8). Therefore, Anderson medium was suited for in-vitro establishment of red sandalwood seeds. However, after seed germination, for further growth of the seedlings, germinated seeds should be transferred to Anderson medium incorporated with 1 g/l of activated charcoal.
How to Cite: Chaturani, G.D.G., Subasinghe, S. and Jayatilleke, M.P., 2009. In-vitro establishment, germination and growth performance of Red Sandalwood (Pterocarpus santalinus L.). Tropical Agricultural Research and Extension, 9, pp.116–130. DOI: http://doi.org/10.4038/tare.v9i0.5411
Published on 30 Dec 2009.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus