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Research Articles

Effects of carbendazim on In vitro micropropagation and flowering of pepper elder herbal plant

Authors:

Abdul Bakrudeen Ali Ahmed,

Ton Duc Thang University, Ho Chi Minh City, VN
About Abdul Bakrudeen
Faculty of Applied Sciences
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Teoh Lydia,

University of Malaya, Kuala Lumpur, MY
About Teoh
Institute of Biological Sciences, Faculty of Science
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Muneeb M. Musthafa,

South Eastern University of Sri Lanka, University Park, Oluvil, #32360, LK
About Muneeb M.
Department of Biosystems Technology, Faculty of Technology
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Vidya Devanathadesikan Seshadri,

Prince Sattam Bin Abdul Aziz University, SA
About Vidya Devanathadesikan
Department of Pharmacology, College of Pharmacy
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Vijay Lobo Vidhyavathi,

PRIST Deemed University, Vallam, Thanjavur, Tamil Nadu, IN
About Vijay Lobo
Department of Biochemistry, Centre for Research and Development (CRD)
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Faiz M. M.T. Marikar

General Sir John Kotelawala Defence University, Ratmalana, LK
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Abstract

The present study reports on application of carbendazim for micropropagation and in vitro flowering of Peperomia pellucida. In vitro multiplication of shoot tips and axillary nodal explants were used in 25-100 mgL-1 carbendazim in 70% alcohol treatment for 5 min in MS (Murashige and Skoog) medium and MS hormone medium The explants were cultured on MS medium supplemented with auxins, cytokinins and additives (Casin hydrolyte and glutamine) used to proliferate the shoots. The maximum number of shoots (21.5/shoot tip) were developed on MS medium supplemented with 2.0 mgL-1 KIN (Kinetin) 0.5 mgL-1 NAA (α- Naphthalene acetic acid), 100 mgL-1 casein hydrolysate and 100 mgL-1 glutamine additives. The highest number of roots per shoot (17.6) and highest rooting frequency (94.2%) were obtained when the adventitious shoots were inoculated on MS medium with 1.5 mgL-1 IAA (Indole-3-acetic acid). The highest number of shoot development (75.4 %) was achieved on MS medium supplemented with 2.0 mgL-1 KIN 2.0 + 0.5 mgL-1 ABA (Abscisic acid) with 5% sucrose. It was found that MS medium devoid of 1.5 mgL-1 IAA promote the root formation and maturation of in vitro flower in P. pellucida. Acclimatized plantlets were transferred to botanical garden and the characters compared with mother plant of P. pellucida.
How to Cite: Ali Ahmed, A.B., Lydia, T., Musthafa, M.M., Seshadri, V.D., Vidhyavathi, V.L. and Marikar, F.M.M.T., 2019. Effects of carbendazim on In vitro micropropagation and flowering of pepper elder herbal plant. Tropical Agricultural Research and Extension, 22(1-2), pp.17–29. DOI: http://doi.org/10.4038/tare.v22i1-2.5484
Published on 30 Jun 2019.
Peer Reviewed

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