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Induced polypoidy under In Vitro conditions and highly efficacious screening of hexaploids in purple coneflower (Echinacea purpurea (L.)Moench)

Authors:

Xiao-Lu Chen ,

Ministry of Agriculture, Danzhou, Hainan, CN
About Xiao-Lu
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences /Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China

College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China
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Dahanayake Nilanthi,

University of Ruhuna, Matara, LK
About Dahanayake
Department of Agricultural Biology, Faculty of Agriculture
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Yue-Sheng Yang,

South China Agricultural University, Guangzhou, Guangdong, CN
About Yue-Sheng
College of Life Sciences
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Dong-Liang Li,

South China Agricultural University, Guangzhou, Guangdong, CN
About Dong-Liang
College of Life Sciences

Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Science, Haikou, Hainan, China
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Jun-Jie Zhang,

South China Agricultural University, Guangzhou, Guangdong, CN
About Jun-Jie
College of Life Sciences
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Qing-Ling Li,

South China Agricultural University, Guangzhou, Guangdong, CN
About Qing-Ling
College of Life Sciences
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Rong Chen,

South China Agricultural University, Guangzhou, Guangdong, CN
About Rong
College of Life Sciences
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Hong Wu

South China Agricultural University, Guangzhou, Guangdong, CN
About Hong
College of Life Sciences
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Abstract

Leaf, petiole, and root explants from in vitro maintained triploid purple coneflower plantlets were treated with120 mg·L-1colchicine for the induction of hexaploid plants. Best result for induction of hexaploid was obtained by treating the triploid root explants with 120 mg·L-1colchicine for 25 days. Regeneration of adventitious buds from the triploid root explants on Murashige and Skoog (MS) medium with 6-Benzyladenine (BA) and Naphthalene acetic acid (NAA) after the colchicine treatment persisted for a time longer than from those untreated. Only slow-growing plantlets in a same genotype-and-treated population were selected and detected to increase the screening efficiency as well as save time and efforts. Chromosome counting confirmed that most early-regenerating and fast-growing buds were triploid, while most late-regenerating and slow-growing buds were hexaploid, screening only those slow-growing regenerated plantlets could increase effectively the hexaploid. In the present study, screening efficiency increased from 21% (detected all plantlets) to 53% (detected only the slow-growing plantlets). On the other hand, hexaploid plants had much larger stomata and more stomatal guard cell chloroplasts. The stomatal guard cell chloroplast number in hexaploid plants has a good linear relationship with those in the diploid, triploid, and the tetraploid progenitors. Results indicate that the colchicine-induced hexaploid could be induced and screened out with high efficiency, making this process worth further exploring in other species as well.
How to Cite: Chen, X.-L., Nilanthi, D., Yang, Y.-S., Li, D.-L., Zhang, J.-J., Li, Q.-L., Chen, R. and Wu, H., 2016. Induced polypoidy under In Vitro conditions and highly efficacious screening of hexaploids in purple coneflower (Echinacea purpurea (L.)Moench). Tropical Agricultural Research and Extension, 19(1), pp.201–215. DOI: http://doi.org/10.4038/tare.v19i1.5385
Published on 18 Mar 2016.
Peer Reviewed

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