Start Submission Become a Reviewer

Reading: Near-optimal levels of 2,4-Dichlorophenoxyacetic acid and indole acetic acid for in-vitro di...

Download

A- A+
dyslexia friendly

Research Articles

Near-optimal levels of 2,4-Dichlorophenoxyacetic acid and indole acetic acid for in-vitro direct regeneration of shoots from sugar-cane (Saccharum spp. Hybrid) leaf tissues

Authors:

D. N. Balagalla ,

Sugarcane Research Institute, Uda Walawe, LK
X close

A. Wijesuriya,

Sugarcane Research Institute, Uda Walawe, LK
X close

N. P. Ranathunge,

University of Ruhuna, Mapalana, Kamburupitiya, LK
About N. P.
Department of Agricultural Biology, Faculty of Agriculture
X close

A. M. M. S. Perera

Sugarcane Research Institute, Uda Walawe, LK
X close

Abstract

Four combinations with two levels of each 2,4-Dichlorophenoxyacetic Acid (2,4-D) (0.2mg/L, 0.5mg/L) and Indole Acetic Acid (IAA) (1mg/L, 4mg/L) together with 0.1 mg/L of Kinetin and 40mg/L of Cysteine Hydrochloride de-termined by testing their 150 combinations for in-vitro direct shoot regeneration from sugarcane (Saccharum spp. Hybrid) leaf tissues were further evaluated using the variety SL 96 328 in a completely randomised layout with 30 replicates to find out their near-optimal levels. Leaf spindle slices of the sugarcane variety SL 96 328 was used as ex-plants for culture in the modified MS medium. After 3 weeks of culture, the survival of ex-plants and the num-ber of shoots directly regenerated from ex-plants were recorded in one-week intervals. The concentration of 0.5mg/L of 2,4-D and 1mg/L of IAA together with 0.1mg/L of Kinetin and 40mg/L of Cysteine Hydrochloride re-generated the highest number of viable shoots from leaf spindle discs after 4 weeks of culture. On average, 21 shoots were obtained from a leaf spindle disc of 2mm thickness and 3mm in diameter obtained from sugarcane plants of 5- to7- month old.
How to Cite: Balagalla, D.N. et al., (2015). Near-optimal levels of 2,4-Dichlorophenoxyacetic acid and indole acetic acid for in-vitro direct regeneration of shoots from sugar-cane (Saccharum spp. Hybrid) leaf tissues. Tropical Agricultural Research and Extension. 18(3), pp.112–116. DOI: http://doi.org/10.4038/tare.v18i3.5334
Published on 26 Nov 2015.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus