Start Submission Become a Reviewer

Reading: Callus formation and organogenesis of tomato (Lycopersicon escu-lentum mill variety Thilina)

Download

A- A+
Alt. Display

Review Articles

Callus formation and organogenesis of tomato (Lycopersicon escu-lentum mill variety Thilina)

Authors:

I. P. Manawadu,

University of Ruhuna, Mapalana, Kamburupitiya, LK
About I. P.
Department of Agricultural Biology, Faculty of Agriculture
X close

Dahanayake Nilanthi ,

University of Ruhuna, Mapalana, Kamburupitiya, LK
About Dahanayake
Department of Agricultural Biology, Faculty of Agriculture
X close

S. G. J. N. Senanayake

University of Ruhuna, Mapalana, Kamburupitiya, LK
About S. G. J. N.
Department of Agricultural Biology, Faculty of Agriculture
X close

Abstract

This study was conducted to investigate the effects of explant source and hormone concentrations on the cal-logenesis, calli growth, plantlet regeneration and rooting of a local tomato variety (Lycopersicon esculentum mill. variety ‘Thilina’). Different combinations of Benzyl Adenine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) were used with hypocotyl, leaf and root explants in a completely randomized design with five replicates to evalu-ate the success in plantlet regeneration. Regenerated healthy shoots sub cultured in to MS medium with various concentrations of Indole-3-butric acid (IBA) for rooting.

 

After one month the weight of fresh callus, number of regenerated shoots and roots were evaluated. Anova (DMRT) test shows there were significant effects at p<0.05 level.

 

Combination of BAP (0.1mgl-1) with 2.4-D (2.0mgl-1) and hypocotyl explant produced the best quality fresh callus in highest weight. The best hormonal combination for shoot regeneration (4 shoots/explant) was 0.1mgl-1 NAA and 0.5mgl-1 Kinetin from callus. Maximum direct regeneration was observed on MS medium containing 0.5mgl-1 Ki-netin, 2.0mgl-1 BAP, 0.1mgl-1 1-Naphthaleneacetic acid (NAA) and 100mgl-1 my-inositol within 15-20 days (4 shoots/explant). Leaf bud revealed to be better explants for direct regeneration. Highest root number per plantlet was observed with 2.0mgl-1 IBA.
How to Cite: Manawadu, I.P., Nilanthi, D. and Senanayake, S.G.J.N., 2016. Callus formation and organogenesis of tomato (Lycopersicon escu-lentum mill variety Thilina). Tropical Agricultural Research and Extension, 17(2), pp.86–94. DOI: http://doi.org/10.4038/tare.v17i2.5311
Published on 01 Nov 2016.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus