Start Submission Become a Reviewer

Reading: Improved culture medium for micropropagation of Aloe vera L

Download

A- A+
dyslexia friendly

Research Articles

Improved culture medium for micropropagation of Aloe vera L

Authors:

NMC Nayanakantha ,

Rubber Research Institute of Sri Lanka, Dartonfield, Agalawatta, LK
X close

BR Singh,

Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar, IN
X close

Anil Kumar

Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar, IN
X close

Abstract

An efficient micropropagation protocol was developed using lateral shoot explants of Aloe vera. Both shoot induction and elongation were better on MS medium supplemented with 4mg/l BAP + 0.2mg/l NAA + 1g/l PVP. All cultures showed shoot regeneration in this medium with 16 shoots/explant. Addi-tion of 1g/l PVP was ineffective for controlling phenolic browning of explants and culture discoloration. However, more adventitious buds (21.5 shoots/explant) developed on MS medium supplemented with 4mg/l BAP + 0.2mg/l NAA + 1g/l PVP+10mg/l citric acid+0.5g/l activated charcoal. Browning of ex-plants was minimized in this medium and elongation of micro-shoots and the growth of the plantlets were also better. Further elongation and rooting of micro-shoots were obtained when sub-cultured on to MS basal medium containing 0.5g/l activated charcoal and 100% of the survival of rooted plantlets was observed after acclimatization. Therefore, the above protocol could be effectively used in rapid mi-cropropagation of elite plants of Aloe vera.  

Key words: Aloe vera, Phenolic browning, Micropropagation, Culture media, Growth hormones

DOI: http://dx.doi.org/10.4038/tare.v13i4.3291

TARE 2010; 13(4): 87-93

How to Cite: Nayanakantha, N., Singh, B. & Kumar, A., (2011). Improved culture medium for micropropagation of Aloe vera L. Tropical Agricultural Research and Extension. 13(4), pp.87–93. DOI: http://doi.org/10.4038/tare.v13i4.3291
Published on 16 Aug 2011.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus