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Anther Culture Potential of Indica Rice Varieties, Kurulu Thuda and BG 250

Authors:

TD Silva ,

Department of Plant Sciences, University of Colombo, LK
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WJ Ratnayake

Department of Plant Sciences, University of Colombo, LK
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Abstract

The anther culture ability of two local indica rice varieties were evaluated by anther plating on N6 and SK-I callus induction media. The traditional rice variety, Kurulu Thuda displayed good anther culture potential with a callus induction frequency of 17.2%. Anthers most suitable for culture were those bearing bi-nucleate pollen. Anther response was relatively poor in the new improved variety, Bg 250 (1.4%). Callus induction was nearly three times higher on N6 medium than on SK-I medium in var. Kurulu Thuda. Shoot regeneration was achieved in var. Kurulu Thuda on SK-II medium, but only from callus induced on N6 medium. From a total of 115 calli transferred from N6 to regeneration medium shoot development occurred in 7 calli, thus limiting the overall anther culture efficiency. Of the calli with morphogenetic competence, 5 gave rise to albino shoots and 2 produced green shoots. After root development the green plantlets were potted in soil. Plant regeneration could not be observed from callus induced in var. Bg 250.

Key words: Anther culture; Callus induction; Indica rice

DOI: 10.4038/tare.v12i2.2789

Tropical Agricultural Research & Extension 12(2): 2009 pp.53-56

How to Cite: Silva, T. & Ratnayake, W., (2011). Anther Culture Potential of Indica Rice Varieties, Kurulu Thuda and BG 250. Tropical Agricultural Research and Extension. 12(2), pp.53–56. DOI: http://doi.org/10.4038/tare.v12i2.2789
Published on 01 Feb 2011.
Peer Reviewed

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