Start Submission Become a Reviewer

Reading: DNA Typing of Dessert Banana Cultivar 'Kolikuttu' ('Silk') Accessions by Microsatellite Markers

Download

A- A+
dyslexia friendly

Short Communications

DNA Typing of Dessert Banana Cultivar 'Kolikuttu' ('Silk') Accessions by Microsatellite Markers

Authors:

HWL Pushpakumari ,

Dept of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, LK
X close

WLG Samarasinghe,

Plant Genetic Resources Centre, Gannoruwa, Peradeniya, LK
X close

SGJN Senanayake

Dept of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, LK
X close

Abstract

 Fourteen Kolikuttu accessions showing morphological variation and Fusarium wilt tolerance were collected. Extracted DNA was subjected to PCR using six SSR primers. PCR products were separated by PAGE and bands were visualized, scored and statistically analyzed using POPGENE 1.32 version to obtain the dendrogram by genetic distance matrix. Out of 19 SSR alleles amplified, 12 were polymorphic resulting in 63.16% loci polymorphism. Based on the dendrogram, three different "Kolikuttu" accessions known as "Athdath Puwalu", the accession collected from Telijjawila, and accession collected from Angunakolapelassa were clustered separately. These three accessions could be considered as different genotypes.  

Key words: DNA Typing; Dessert Banana; Cultivar Kolikuttu

DOI: 10.4038/tare.v12i2.2801

Tropical Agricultural Research & Extension 12(2): 2009 pp.113-116
DOI: http://doi.org/10.4038/tare.v12i2.2801
How to Cite: Pushpakumari, H., Samarasinghe, W. & Senanayake, S., (2011). DNA Typing of Dessert Banana Cultivar 'Kolikuttu' ('Silk') Accessions by Microsatellite Markers. Tropical Agricultural Research and Extension. 12(2), pp.113–116. DOI: http://doi.org/10.4038/tare.v12i2.2801
811
Views
1148
Downloads
Published on 01 Feb 2011.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus